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We developed and evaluated the specificity and sensitivity of a loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne pathogen Vibrio parahaemolyticus.A pair of outer primers and a pair of inner primers was specially designed for recognizing six distinct sequences on the target invA gene.By the detection system,241 bp target DNA was amplified and visualized as ladderlike pattern of bands on agarose gel within 60 rain under isothermalconditions at 65℃.