【摘 要】
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Various super-resolution(SR)techniques aiming at breaking the diffraction barrier have sprung up in past decades(1),such as stimulated emission depletion mi
【机 构】
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Peking University,Beijing 100871
【出 处】
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2014年上海市研究生学术论坛:生物医疗仪器
论文部分内容阅读
Various super-resolution(SR)techniques aiming at breaking the diffraction barrier have sprung up in past decades(1),such as stimulated emission depletion microscopy(STED),reversible optically linear fluorescence transitions microscopy(RESOLFT),structured illumination microscopy(SIM),photoactivated localization microscopy(PALM)and stochastic optical reconstruction microscopy(STORM),superresolution optical fluctuation imaging(SOFI).etc.Among these SR techniques,switchable fluorophores play a crucial impact on ensuring admirable implementation(2).Recently,we developed a unique beneficial reversibly switchable fluorescent protein,mGeos2-VES.By taking advantage of their innate optical and biological merits,such as monomeric structure,high brightness,high photostability and excellent fusion for biological imaging in living cells,we successfully achieved SOFI and PALM multi-modality SR images of U2OS living cells expressing lifeact fused with mGeos2-VES.
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