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Metabolic profile of bile acids was used to evaluate hepatoprotec tion in Nrf2/ARE Oxidation/chemical stress defense pathway caused by Isoliquiritigenin (ISL) based on analysis of 16 bile acids in mice hepatic tissue.High performance liquid chromatography coupled with quadrupole mass spectrometry (HPLC-MS/MS) was applied to determine the contents of various kinds of endogenous bile acids including free bile acids, taurine conjugates and glycine conjugates.Luciferase reporter gene assay, screening licorice-derived compounds targeting Nrf2/ARE signal pathway, showed that ISL was capable of activating Nrf2-driven gene expression.