Background: Based upon Genome-Wide Association Study involving a total of 575 broilers selected for abdominal fat content (unpublished data)in our lab, the mutation rs14135995, which was located within DNAJB6 gene, was identified extremely significantly associated with abdominal fat weight and abdominal fat percent.Therefor, DNAJB6 gene was chosen as candidated gene in order to lay a foundation for further research on the regulation of development of chicken abdominal fat by bioinformatics and tissue expression profile analysis in this study.Methods: The tissue expression profile analysis of DNAJB6 gene in fifteen kinds of tissues including Abdominal Fat, Liver, Pectoralis Muscle, Leg Muscle, Heart, Gizzard, Gizzard Fat, Proventriculus, Ileum, Duodenum, Jejumum, Brain, Testis, Kidney, Spleen tissues in the generation 14th of the chicken divergently selected for abdominal fat content of Northeast Agricultural University (NEAUHLFG14) were studied using quantitative RT-PCR.For each line, a total of 4 male birds were used in present study.Bioinformatics analysis was studied using bioinformatics databases.Results: The results suggested that the gene was located on chromosome 2, the full length was 4 9720 bp, and the length of cDNA was 2 620 bp, and included 10 exons and 9 introns, encoded a protein containing 326 amino acid residues, of which pi was 8.86, molecular weight was 36.68 kD.A CpG island with the probable length of 1 kb and transcriptional binding sites of CREB, Spl, AP2, GATA and so on were found in the region of promoter.A DnaJ domain was included in DNAJB6 protein, there was no signal peptide and transmembrane domains, and the protein was nearest to that of Bos Taurus in molecular evolution.DNAJB6 gene expressed widely in these fifteen tissues, and it was the highest in the Abdominal Fat and Gizzard Fat tissues.Conclusions: These results laid a foundation for further research on DNAJB6 gene and its regulation mechanism to abdominal fat of chicken .