Zebrafish (Danio rerio), first established as a model to study embryonic development,has rapidly emerged as a popular vertebrate model organism in last two decades.In China, zebrafish was adopted in lab research in early 1990s, and the zebrafish research community has been rapidly growing since 2000.To meet the growing needs of zebrafish strains and technical support, in October, 2012, the China Zebrafish Resource Center (CZRC, website: http://zfish.cn) was established at the Institute of Hydrobiology (IHB), Chinese Academy of Sciences (CAS) in Wuhan.After three years development, CZRC has maintained more than 800 mutant and transgenic zebrafish lines, and a number of DNA plasmids and cell lines till now.In addition to providing the regular fish resources, CZRC also provides technical training and technical services, i.e., gene knock-out and transgenic services.In 2013, 40 labs in China started the Zebrafish All Gene Knock-out Project (ZKO Project) for Chromosome 1.The final goal of ZKO project is to knock-out all the 1333 genes on zebrafish chromosome 1 using CRISPR/CAS9 system.Up to now, more than 900 genes have been successfully knocked out.CZRC is the project coordinator, and all mutant lines built in the project will be verified and preserved by CZRC and published as open resources to the whole research field.With the progress of ZKO project, CZRC will expand its fish stocks to 2000 lines by the end of 2015, and it is likely to become the largest zebrafish resource center in Asia.