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Understanding the performance of nanoparticles in a biological context is important for guiding their biomedical applications.In this talk,we will explore studies on the interactions of nanostructures with biomolecules and biological systems using high-throughput proteomics techniques:(1)interaction of gold and silver nanoparticles with human plasma;(2)analysis of Abraxane sensitive and resistant lung adenocarcinoma cell;(3)antitumor mechanism of self-assembled polymer-KLAK peptide conjugates.