Objective: Tissue engineered meniscus offers a possible solution for the issue of regeneration and replacement to solve meniscus injury.However, the non-uniform distribution and declined proliferation of seeded cells on scaffolds are continues to be a scientific and translational challenge.This study was designed to investigate the efficiency and effect of seeding techniques (static seeding, injection seeding, centrifugal seeding and vacuum seeding).Methods: Cell-seeding efficiency, survival, distribution, and long-term proliferation on scaffolds were quantitatively evaluated.Cell adhesion was compared via cell-binding kinetics.Cell viability and morphology were assessed by using fluorescence staining.Combined with the reconstructed three-dimensional image, the distribution of seeded cells was investigated.The Cell Counting Kit-8 assay and DNA assay were employed to assess cell proliferation.Results: Cell-binding kinetics was improved via centrifugal or surface static loading compared to injection or vacuum seeding methods.Seeded cells were homogeneously distributed throughout the scaffold by using centrifugation;Moreover, the centrifuge group showed more seed cells in deep layer (300-500um) compared with the surface static and injection seeding.The long-term proliferation in centrifugal group was significantly higher than the other groups.Conclusions: The centrifugal seeding method could improve cells distribution and proliferation on three-dimensional scaffolds, which provides insight into the design of future experiments.