In this study,mature zygotic embryos and plant growth regulators to regenerate were used plantlets of the bamboo species Dendrocalamus hamiltonii.Incubating explants in Murashige and Skoog (MS) medium supplemented with 1.0-3.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) induced callus formation.Optimal shoot differentiation and subsequent shoot growth was also obtained in the MS medium supplemented with 2 mg/L benzyladenine (BA),1 mg/L kinetin (KT),and 1 mg/L naphthaleneacetic acid (NAA).Root induction was enhanced by 5 mg/L indole-3-butyric acid (IBA).Histological analysis demonstrated that somatic embryogenesis and organogenesis were induced during callus initiation,shoot differentiation,and development of plantlets from the mature zygotic embryos.Our data provides a useful means for bamboo plant regeneration.