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Cells are the fundamental unit of life,and communication between cells by release of specific chemical messengers plays crucial roles in biological process.Electrochemical methods based on the microelectrode have played important roles in real-time monitoring of these events at single cell level.However,further application of this technique needs multifunctional electrochemical sensors with higher performance.Furthermore,neuronal communication occurs inside synaptic cleft with very small size(< 100 nm),while real-time monitoring of vesicular exocytosis and its dynamics inside neuronal synapse is still a great challenge.Recently,We developed some multifunctional(e.g.highly sensitive,highly selective,reusable,cell compatible and stretchable)electrochemical sensors with high performance,and monitored various kind of chemical messengers release from single cells in real time.[1-4] To realize monitoring of neurotransmitter release from inside individual synapse,we fabricated a novel finite conical nanoelectrode and developed a nanoelectrode amperometric method for probing into single synapse.[5] Further combining with microfluidic devices allows amperometric detection of individual neurotransmitter release events as well as recording of postsynaptic potential,[6] which provides direct evidences that complex exocytosis is a major mode of vesicle fusion inside synapse.