Nucleotide-binding site (NBS) and leucine-rich repeat (LRR) class gene can be obtained from plant genome based on PCR-based strategy according to conserved motif of NBS domain. In order to clone R class gene responsible for binding pathogen virulence factors in mango, we designed six pairs of degenerate primers; the left primer corresponding to protein sequence of P-loop of NBS domain and the right primer from kinase 2 of NBS domain. Twenty three clones were isolated from the PCR fragment and then sequenced. Their homologous extent between RGAs varied from 33.3％ to 98.5％. Among them, sixteen DNA sequence can be translated into amino sequence. The high homolog in protein sequence of P-loop and kinase 2 of NBS domain between RGAs isolated in the study and characterized R gene indicate these cloned sequence belong to the NBS-LRR gene family. Moreover, these RGAs can be classified into non-TIR-NBS-LRR gene family because of only tryptophan (w) occurs in the last residual of kinase-2 of all RGAs isolated in the investigation. From our result, we can conclude that R NBS-LRR genes possessed a high variation range in mango genome, which is advantage for mango to recognize different pathogen virulence factors.