【摘 要】
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Genetically modified (GM) livestocks have the potential to contribute to improving the environment and human health, with consumption of fewer resources and reduced waste production.However, the trans
【机 构】
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Key Laboratory of Farm Animal Genetic Resources and Utilization & Inspection Test Center for Biosafe
论文部分内容阅读
Genetically modified (GM) livestocks have the potential to contribute to improving the environment and human health, with consumption of fewer resources and reduced waste production.However, the transgene process also poses risks.The safety assessment and control of transgenic animal products have drawn wide attention, and the relevant regulations and technology are being developed.Quick testing technology plays a significant role in on-site and customs sampling.Nowadays, loop-mediated isothermal amplification (LAMP) was widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency and specificity.In this study, a specific, the sensitive detection system for detecting sFAT-1 transgenic pigs was designed.A set of 6 primers including 2 loop primers was designed for the target sequence.The DNA samples were amplified in less than 1 hour at the optimized temperature and detecting by both Nephelometer LA-320c and unaided eyes directly adding calcein.The detection limit of sFAT-1 LAMP was as low as 1.26 ng/μL.Furthermore, blind tests of transgenic and non-transgenic DNA samples were all correctly detected.Hence, the results in this study demonstrated that LAMP is a very useful tool for transgenic detection.
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