【摘 要】
：
An extracellular β-xylosidase from the thermophilic fungus Paecilomyces thermophila J18 was purified 31.9-fold to homogeneity with a recovery yield of 2.27
【机 构】
：
Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural
【出 处】
：
2007 Enzyme Engineering Conference(国际酶工程学术会议)
论文部分内容阅读
An extracellular β-xylosidase from the thermophilic fungus Paecilomyces thermophila J18 was purified 31.9-fold to homogeneity with a recovery yield of 2.27% from the cell-free culture supematant by ammonium sulfate precipitation,DEAE-52 and Q-Sepharose Fast Flow chromatographies.It appeared as a single protein band on SDS-PAGE with a molecular mass of approx 53.5 kDa.The molecular mass of β-xylosidase was 51.8 kDa determined by Superdex 75 gel filtration.The enzyme was a glycoprotein with a carbohydrate content of 61.5%.
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