壳寡糖在刚健芦荟组培快繁上的应用研究

来源 :湖北省细胞生物学学会2015年会员代表大会暨学术研讨会 | 被引量 : 0次 | 上传用户：fanfan19860303

【摘要】

：

　　壳寡糖(Oligochitosan)由甲壳素(几丁质)脱乙酰化的产物壳聚糖降解获得，是由2～10个氨基葡糖通过β-1-4糖苷键连接而成的低聚糖，也是天然糖中唯一大量存在的碱性氨基多糖.本

【作者】

：

匡银近雷海霞张利芳

【机构】

：

湖北工程学院生命科学技术学院,湖北孝感432000

【出处】

：

湖北省细胞生物学学会2015年会员代表大会暨学术研讨会

【发表日期】

：

2015年10期

【关键词】

：

壳寡糖胡萝卜组织培养愈伤组织

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　　壳寡糖(Oligochitosan)由甲壳素(几丁质)脱乙酰化的产物壳聚糖降解获得，是由2～10个氨基葡糖通过β-1-4糖苷键连接而成的低聚糖，也是天然糖中唯一大量存在的碱性氨基多糖.本文以刚健芦荟(Aloe nobilis)茎切段为材料，研究了壳寡糖在植物组培中的应用.结果表明：壳寡糖作为一种植物生长调节剂，可诱导刚健芦荟茎段愈伤组织的形成，促进不定芽的分化和生根，同时还可防止外植体褐变.诱导芽分化培养基MS+6-BA1.0mg/L附加4.0mg/L壳寡糖有利于提高刚健芦荟茎段愈伤组织的诱导率；继代增殖培养基MS+6-BA1.0mg/L+NAA 0.5mg/L附加4.0mg/L壳寡糖可诱导试管苗的增殖率达到6倍以上；生根培养基1/2MS+NAA 0.5mg/L附加2.0mg/L壳寡糖促使试管苗根系发达.

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壳寡糖在刚健芦荟组培快繁上的应用研究

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