【摘 要】
：
Fruit development is a complex process involving dramatic changes in the transcriptional architecture.Sorbitol and sucrose serve as main photosynthetic end-product in apple.We report here a comprehens
【机 构】
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Institute for Horticultural Plants,China Agricultural University,Beijing 100193,China;Department of
【出 处】
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2015 International Conference on Fruit Quality Biology (第二届果
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Fruit development is a complex process involving dramatic changes in the transcriptional architecture.Sorbitol and sucrose serve as main photosynthetic end-product in apple.We report here a comprehensive analysis of transcriptome dynamics of A6PR antisense apple fruit.With suppressed A6PR expression the fruit metabolism network has changed a lot.Marked changes were found in the expression of genes involved in metabolic pathways,plant hormone signal transduction.Detailed analyses of enriched KEGG pathways indicate that the effect of varied sorbitol levels on the cell wall metabolism is associated with both altered ethylene biosynthesis genes and carbohydrate metabolism.Furthermore,our results showed sorbitol may affect the splicing ways of genes which involved in metabolic processes.To uncover sorbitol-specific network connections and demonstrate how differential network connectivity can be used to identify the functions of sorbitol,we identified and visualized modules of coexpressed genes to explore differences in these modules between the antisense and control lines.These comparisons provide a systems-level context which suggested a large difference in connectivity for those hub genes,reflect decreased sorbitol supply affecting genes and its neighbors and some specificly enriched in hormone response,stress related genes.Together,the results provide a valuable resource that reflects the sorbitol may directly play a role in abiotic stresses and hormone regulatory processes.
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