论文部分内容阅读
Objective To observe the changes in the function of bone marrow-derived endothelial progenitor cells (EPCs) after bone-marrow mobilization, and investigate the possible mechanism of autologue bone-marrow stemceils implantation improving ischemic limb disease after bone-marrow mobilization.Methods Lewis ratswere enrolled in bone marrowmobilization group and control group.In mobilization group, the bone marrowof each ratwas stimulated byinjection of rhG-CSF.Mononuclear cells were harvested from bone marrow and cultured in EBM-2 medium.After 7-day culture, EPCs were characterized.The adherent function of EPCs was determined by counting th e number of adherent EPCs.