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Objective:Gastric bypass results in the rapid resolution of type 2 diabetes.However, the underlying mechanisms remain unclear.This study was to observe the expression of hepatic IRS-2 and GLUT-2 of type 2 diabetic rats after Duodenal-Jejunal Exclusion (DJE), and explore the possible mechanism of insulin resistance improved.Methods: Healthy male SD rats were randomly divided into type 2 diabetes-operation group (DO group), type 2 diabetes-control group (DC group) ; normal-operation group (NO group) and normal-control group(NC group).Plasma glucose concentration, insulin were measured respectively at fasting condition in preoperative and postoperative 1 week, 2 weeks, 4 weeks and 8 weeks.Quantitative Insulin Sensitivity Check Index (QUICKI) were measured respectively in preoperative and postoperative 8 weeks.Protein of hepatic IRS-2 and GLUT-2 were detected by Western Blot and mRNA of hepatic IRS-2 and GLUT-2 were detected by RT-PCR in postoperative 8 weeks.Results:The fasting blood glucose of DO group rats decreased from the preoperative (20.21 ±2.14) mmol/L to (8.50 ± 2.19) mmol/L (P < 0.05) at 8th week after DJE respectively.The change of QUICKI: DO group had increased dramaticly, from preoperative 0.43 ± 0.02 to 0.55 ± 0.05 in postoperative 8 weeks (P < 0.05).Compared with the DC group, in postoperative 8 weeks in the DO group, the IRS-2 protein,the GLUT-2 protein and the IRS-2 mRNA , the GLUT-2 mRNA were significantly increased respectively.Conclusion: DJE was effective in up-regulating the expression of hepatic IRS-2 and GLUT-2 in the hepatic insulin signaling pathway of type 2 diabetic rats, and meanwhile the insulin sensibility was improved.