【摘 要】
：
Interspecific mycelial interactions between white rot fungi are always accompanied by increased production of laccase.In this study,the potential of white rot fungi Dichomitus squalensfor enhancing la
【机 构】
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Research Center of Bioenergy and Bioremediation, College of Resources and Environment,Southwest Univ
【出 处】
：
第二十次全国环境微生物学学术研讨会
论文部分内容阅读
Interspecific mycelial interactions between white rot fungi are always accompanied by increased production of laccase.In this study,the potential of white rot fungi Dichomitus squalensfor enhancing laccase production during interaction with two other white rot fungi Trametes versicolor or Pleurotus ostreatuswas identified.To probe the induction mechanism of laccase and the role of laccase played during the combative interaction,we analyzed the laccase induction response to stressful conditions during fungal interaction related to the differential gene expression profile.We further confirmed the expression patterns of 16 selected genes by qRT-PCR analysis.We noted that many differential expression genes (DEGs) encoding proteins were involved in xenobiotics detoxification and ROS generation or reduction,including aldo/keto reductase,glutathione S-transferases,aldehyde dehydrogenase,cytochrome P450 enzymes,alcohol oxidases,GroEs-like protein and laccase.Furthermore,many DEG-encoding proteins were involved in antagonistic mechanisms of nutrient acquisition and antifungal properties,including glycoside hydrolase,alpha/betahydrolase and terpenoid synthases.DEGs analysis effectively revealed that laccase induction was likely caused by protective responses to oxidative stress and nutrient competition during fungal interspecific interaction.
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