【摘 要】
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Polycyclic aromatic hydrocarbons (PAHs) and dioxin-likecompounds,including sulfur,nitrogen,and oxygen heterocycles,are widespread and toxic environmental pollutants.Microbial decomposition of these po
【机 构】
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State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai
【出 处】
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第二十次全国环境微生物学学术研讨会
论文部分内容阅读
Polycyclic aromatic hydrocarbons (PAHs) and dioxin-likecompounds,including sulfur,nitrogen,and oxygen heterocycles,are widespread and toxic environmental pollutants.Microbial decomposition of these pollutants is essential for processes of bioremediation of contaminated sites as well as the Earths carbon cycle.Cells of the gram-negative bacterium Pseudomonas putida strain B6-2 grown in the presence of biphenyl are able to cometabolic PAHs and dioxin-like compounds.Here,we determined the complete genome sequence ofPseudomonas putida B6-2 and investigated the dynamic response of P.putida B6-2 to biphenyl and mixture of biphenyl (BP),dibenzofuran (DBF),benzothiophene (DBT),carbazole (CA) using genomic,transcriptome,and proteome analyses.The genome comprised a circular chromosome DNA (6,377,291),is devoid of plasmids and contains 5,832 coding sequences.A total of 1,241 proteins were identified by proteomic analysis when using biphenyl as the sole carbon source,of which 254 proteins were notably altered (114 up-regulated and 140 down-regulated).Genetic analyses and transcriptome analyses revealed the transformation of biphenyl,the degradation of benzoate and 4-hydroxybenzoate,and the degradation of salicylate via catechol 2,3-dioxygenation.Weobserved dynamic changes in transcriptional level during BP or mixture of BP/CA/DBT/DBF,including metabolic pathway,chemotaxis,and transporters potentially involved in adaptation to the PAHs.
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