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背景:在老化过程中,脑内环境改变可引起脑内神经干细胞增殖能力改变。脑内神经干细胞与衰老和退行性神经病变疾病密切相关,增殖能力与年龄存在负相关,但以快速老化小鼠为衰老模型的相关研究未见报道。目的:比较快速老化与正常老化小鼠嗅球、海马、皮质神经干细胞增殖的差异。方法:分别取6只快速老化小鼠(SAMP8)和6只正常老化小鼠(SAMR1)的嗅球、海马、皮质组织,在固定、冰冻切片后,运用Ki-67/Nestin免疫荧光双标检测3个脑区的神经干细胞增殖情况。免疫荧光双标在荧光显微镜下通过Leica Qwin v3采图,在40倍物镜和10倍目镜下采图,每一张切片随机选取5个相邻视野,通过Image-pro-Plus软件完成图像分析。结果与结论:正常老化小鼠和快速老化小鼠均有神经干细胞增殖现象,但二者存在差异,其差异主要表现在海马和嗅球两个脑区(P<0.05)。提示快速老化可能会导致海马、嗅球神经干细胞增殖能力降低。
Background: During the aging process, changes in the environment in the brain can cause changes in the proliferation of neural stem cells in the brain. Neural stem cells in the brain are closely related to the diseases of aging and degenerative neuropathies, and the proliferation ability is negatively correlated with the age. However, no studies have been done on the aging model of the mice aged rapidly. OBJECTIVE: To compare the differences of proliferation of neural stem cells in the olfactory bulb, hippocampus and cortex between the fast aging and normal aging mice. Methods: Olfactory bulb, hippocampus and cortex tissues of 6 fast-aging mice (SAMP8) and 6 normal aging mice (SAMR1) were harvested, and fixed and frozen sectioned respectively. Ki-67 / Nestin immunofluorescence double- A brain area of neural stem cell proliferation. Immunofluorescence double labeling was taken by Leica Qwin v3 under a fluorescence microscope under a 40x objective and a 10x eyepiece. Five adjacent fields were randomly selected for each section and image analysis was performed by Image-pro-Plus software. RESULTS AND CONCLUSION: Normal aging mice and rapidly aging mice all have neural stem cell proliferation phenomenon, but there are differences between the two groups. The difference is mainly in the two brain regions of hippocampus and olfactory bulb (P <0.05). Prompted rapid aging may lead to hippocampal, olfactory bulb, neural stem cell proliferation decreased.