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In vertebrates,hematopoietic stem cells(HSCs)form during embryogenesis and maintain our blood system throughout life.Using a cytochemical staining(Sudan Black B)in ENU-mutagenized zebrafish offsprings,we found a mutant line in which SB+ cells were absent.Mapping results suggested that the causative gene is poc1a.Poc1a(POC1 centriolar protein homolog A)is a member of the Poc1(Protein of Centriole 1)family,conserved centriole proteins in vertebrate that are required for basal body organization and stability.However,its role in hematopoietic development is unknown.poc1a starts to be expressed in hematopoietic cells at early embryogenesis in zebrafish.In the poc1a-deficient zebrafish embryos,poc1a acts as a novel factor essential for neutrophil maturation in controlling the activity of myeloperoxidase,but does not affect the expression of mpx.Using differential interface contrast(DIC)in the mutant embryos,less granules in the neutrophils are detected,of which morphology are normal.We showed that in the mutant embryos,both definitive erythroid and lymphoid cells are reduced.In addition,HSCs reduce slightly,demonstrating poc1a is required for HSC development.