【摘 要】
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As one of the mostly used nanomaterials (NMs), silver nanoparticles (AgNPs) are mainly applied in several consumer products, including personal care products, laundry additives, home appliances, paint
【机 构】
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Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences,Chongqing, 4007
【出 处】
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2016(第二届)毒性测试替代方法与转化毒理学(国际)学术研讨会暨有害结局路径(AOP)与风险评估培训会议
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As one of the mostly used nanomaterials (NMs), silver nanoparticles (AgNPs) are mainly applied in several consumer products, including personal care products, laundry additives, home appliances, paints and textiles particularly for their antiseptic and nanotechnology properties.The wide utilization of AgNPs will lead to massive release of AgNPs into aquatic environment, and pose a threat to aquatic organisms and humans health.Silver nanoparticles (AgNPs) was suspected to lead oxidative stress responses, impair mitochondrial function and result in cell apoptosis.However, less is known about its reproductive toxicology in aquatic organisms.Here, three-month-old zebrafish (Danio rerio) were exposed to AgNPs with different concentrations (0, 10, 33 and 100 μg/L) for five weeks, then the impact on reproduction and the underlying mechanism were investigated.Exposure to 33 and 100 μg/L AgNPs significantly decreased the fecundity of female zebrafish, accompanied by increasing apoptosis cells on ovarian and testicular tissue by using TUNEL assay.To explore the mechanism of apoptotic pathway after exposure to AgNPs, transcription levels of several genes related to mitochondrion-mediated apoptosis pathway, such as bax, bcl-2, caspase-9 and caspase-3 were examined.The results indicated that the apoptosis of germ cell in zebrafish gonad tissue induced by AgNPs was via mitochondrion-mediated apoptosis pathway, which in turn, could cause adverse effects on reproduction in zebrafish.
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