【摘 要】
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In artificial insemination, semen quality is considered to be closely related to the pregnancy rate and ratio of embryo development into fetus.The aim of th
【机 构】
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EmbryoBiotechnologyandReproductionLaboratory,InstituteofAnimalScience,ChineseAcademyofAgriculturalSc
论文部分内容阅读
In artificial insemination, semen quality is considered to be closely related to the pregnancy rate and ratio of embryo development into fetus.The aim of this study was to evaluate the quality of sex-sorted vs unsorted semen in IVF.Semen was collected from seven bulls (A-G).Following IVF, the fertilization rate, cleavage rate, blastocyst rate, total cells number (TCN), ICM/TCN ratio and apoptotic cell ratio (ACR) were determined.The results showed that except bull C, the unsorted semen yielded a significantly higher IVF rate than sex-sorted semen (p<0.05).A significantly higher percentage of embryos reached the two-cell stage using unsorted semen than the matched sex-sorted semen from the seven bulls (both p<0.05), and the blastocyst stage from bulls A, C, D, E and G.However, there was no significant difference in the ICM/TCN ratio between the IVF blastocysts and the sexed blastocysts from six bulls.Also, there was no significant difference in the expression of IGF1 gene in bovine embryos produced with sorted or unsorted semen.However, the GDF9 gene expression in embryos from bull F was significantly higher than in the others (p<0.05).No significant difference was found in ACR and Bcl-2 expression among the seven bulls, while there was a difference of Bax expression in the sorted sperm of only one bull.Conclusively, the quality of unsorted semen was generally better than that of sex-sorted as to embryo productivity in vitro.Individual differences in fertility were observed among different bulls.
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