There is a large content of multi-umani substance in the pre-cooking fluid of canned mushroom,so its significantly to study the compositions of umani substance and exploit it into mushroom seasoning.Related combinations of mushroom were studied:umani substance was separated by chromatographer linked sensory evaluation; compositions of amino acid from pre-cooking fluid were analyzed by amino acid automatic analyzer; content and compositions of umani-nucleotide were studied by High Performance Liquid Chromatography (HPLC).