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Methods Cell viability was assessed by MTT assay.AO/EB,Hoechst 33258 staining assay,cell colony formation and migration were determined to evaluate the inhibition effect.The mechanism of action was further studied by cell cycle and Annexin V-FITC/PI double staining detection of flow cytometry,following the determination of ROS production,△Ψm,western blotting and qRT-PCR assay.