【摘 要】
：
Background MicroRNAs(miRNAs)are highly conserved,short non-coding RNAs that regulate gene expression at the posttranscriptional level.Although many miRNAs are identified in muscles and muscle cells,th
【机 构】
：
College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China;Ch
论文部分内容阅读
Background MicroRNAs(miRNAs)are highly conserved,short non-coding RNAs that regulate gene expression at the posttranscriptional level.Although many miRNAs are identified in muscles and muscle cells,their individual roles are still not fully understood.Result In the present study,we investigated a muscle highly-expressed miRNA,miR-127-3p,in C2C12 myoblasts and tissues of goats with different muscle phenotypes(Boer vs.Wushan black goats).Our results demonstrated that(1)miR-127-3p was extensively expressed in tissues of goats;(2)miR-127-3p was higher expressed in muscle,spleen,heart and skin in the muscular goats(Boer goats)than the control(the Wushan black goats).Then we further characterized the dynamical expression of miR-127-3p,MyoD,MyoG,Myf5,Mef2c and Myosin in the proliferating and differentiating C2C12 myoblasts at day of 0,1,3,5,and 7 in culture mediums.Especially,we found that miR-127-3p was significantly higher expressed in the proliferating than differentiating cells.Conclusion Our findings suggest that miR-127-3p probably play roles in the proliferation and differentiation of myoblasts,which further underlies regulation of muscle phenotype in goats.
其他文献
目的：本试验是为了考察饲粮中添加鸡血浆蛋白粉(SDCP)替代猪血浆蛋白粉(SDPP)对断奶仔猪生产性能、消化功能及肠道微生物菌群的影响.方法：试验选取144头25d、体重6.43±0.39kg的断奶仔猪,随机分为4个组,分别为:COn组(对照);SDPp组(含5％猪血浆蛋白粉);SDPP+SDCp组(含2.5％猪血浆蛋白粉和2.5％鸡血浆蛋白粉);SDCp组(含5％鸡血浆蛋白粉).第28d试验结束
本试验旨在研究过瘤胃赖氨酸(rumen protected lysine,RPLys)和过瘤胃蛋氨酸(rumen protected Methionine,RPMet)对肉牛生长性能的影响.选择20头年龄相近且健康状态良好的肉用水牛,随机分成4组,每组5头.在相同饲养管理条件下,每组添加不同量的RPLys和RPMet,结果发现添加过瘤胃氨基酸的3个组平均日增重分别为0.82kg、0.87kg和0.
目的：本研究旨在探讨饲料补充苹果果胶寡糖(apple pectic oligosaccharide,APOS)是否可以改善断奶大鼠抗氧化能力、空肠粘膜形态结构和盲肠菌群.方法：试验选取24只健康的断奶Wistar大鼠,按照体重相近的原则分为2个处理,分别饲喂基础饲粮和添加800Mg/kgAPOS的饲粮,试验期为14d.结果：饲粮补充APOS显著提高了断奶大鼠平均日增重和平均日采食量(P＜0.05)
目的：本研究旨在研究不同来源α-半乳糖苷酶对肉鸡的肠道微生物区系和肠道组织形态等指标的影响.方法：选用864只1日龄AA肉仔鸡随机分为4个处理,每处理设12个重复,每重复18只肉仔鸡,公母各半,各处理组之间起始体重差异均不显著(P＞0.05).试验期共42d,分前期(1-21d)和后期(22-42d)两期进行.结果：试验结果表明,转α-半乳糖苷酶基因玉米未对肉仔鸡的肠道造成破坏或不良影响,且转基因
目的：本试验旨在研究不同饲粮蛋白质水平对育雏期北京鸭苏氨酸需要量的影响.方法：试验采用2×6二因子完全随机试验设计,包括2个饲粮粗蛋白质水平(17.5％和20.5％),6个饲粮苏氨酸添加水平(0、0.06％、0.12％、0.18％、0.24％和0.30％).将576只1日龄健康雄性北京鸭随机分为12个处理组,每处理组6个重复,每重复8只鸭,试验期为0～14日龄.结果：饲粮添加苏氨酸可以提高北京鸭的
目的：本试验旨在研究转α-半乳糖苷酶玉米对肉仔鸡生长性能及饲料养分的影响,并以微生物源α-半乳糖苷酶作为正对照.方法：选用864只1日龄AA肉仔鸡随机分为4个处理,每处理设12个重复,每重复18只肉仔鸡,公母各半,各处理组之间起始体重差异均不显著(P＞0.05).试验期42d,分前期(1-21d)和后期(22-42d)两个阶段.结果：各试验组间肉鸡日增重、采食量和料重比差异不显著(P＞0.05);
An experiment was conducted to investigate the effect of arginine on feed intake regulation.One hundred and twenty six 1-day-old male White Pekin ducks(Anas platyrhynchos domestica)were randomly were
目的：本试验旨在探讨日粮添加不同剂量L-茶氨酸(L-theanine)对育肥猪生长性能、血液生化及免疫因子水平的影响.方法：选择育肥猪48头,随机分4个组,每组3个重复,每个重复4头猪.对照组饲喂普通全价日粮,试验组在普通日粮基础上分别添加200mg/kg(试验1组)、500mg/kg(试验2组)和1000mg/kg(试验3组)L-茶氨酸.试验期37d.结果:试验3组的终末重、平均日增重(ADG)
本试验旨在研究不同水平物理有效中性洗涤纤维(peNDF)饲粮对热应激条件下泌乳中期奶牛采食量与产奶性能的影响.选取25头健康、146±25DIM、2.3胎、平均泌乳量为18.3±1.2kg/d的中国荷斯坦奶牛,根据胎次、泌乳天数、泌乳量将奶牛随机分为A、B、C、D和E5个处理组,每个处理5个重复,每个重复1头牛.5个处理组的peNDF水平分别为23.77％,24.20％,24.28％,24.65％
Background Long noncoding RNAs(lncRNAs)play roles in almost all biological processes; however,their function and profile in skin development and pigmentation is less understood.In addition,because lnc