Toxoplasma gondii is an obligate intracellular protozoan,that can infected almost any nucleated cells of warm:blooded animals,including humans.Prior to invasion of host cell,tachyzoites gliding on the host cells surface were mediated by adhesin factors released from apical organelle —microneme.Identifying the host proteins interacting with microneme protein will increase our understanding about parasite gliding and invasion into host cells.In this study,Universal Mouse Library were screened with MIC3:bait by yeast two:hybrid technique.Using mating screened procedure and excluding false positive,we identified eight host proteins that had not previously been reported to interact with TgMIC3 such as Supervillin (Svil),PHD finger protein 7 (Phf7),dickkopf:related protein 2 (DKK2),Transmembrane protein 100 (Tmem100),spermatogenesis:associated protein 3 (Spata3) isoform 2 and isoform 3,uncharacterized protein LOC210904 and uncharacterized protein LOC72128.These host proteins are associated with cytoskeleton organization,actin binding,transcriptional regulation,signaling transduction and cell adhesion.Our data strongly support the hypothesis that the microneme protein MIC3 is involved in several complex invasion steps such as adhesion,gliding motility and regulation of host actin rearrangement.