Objective: This study investigated the antitumor responses by necrosis H22cell lysate stimulated peritoneal macrophages in mice.Methods: 40 male and female BALB/c mice were randomly divided into four groups, each group consisting of 10animals (with 5 males and 5 females in), which were vaccinated with sterile saline, heat inactivated H22 hepatocarcinoma cells, liquid paraffin-induced peritoneal macrophages and necrosis H22 cell lysate stimulated peritoneal macrophages respectively in the left hind limb.Then H22 cells were inoculated to each mouse by subcutaneous injected in leR fore limb.Mice were euthanized on day 28 after injection of the H22 cells and tumor tissue were totally excised from the animal.Peripheral blood mononuclear cells (PBMC)and Spleenocytes were used as effector cells for cytotoxicity assay.Cytokine production by spleen cells were measured by ELISA and Western blot analysis.Results: the tumor formation rate, volume and weight of tumors in necrosis H22 cell lysate stimulated peritoneal macrophages vaccinated mice are significantly less than control groups.The tumor cells injured rate and the activity of LDH in the supematant in necrosis H22 cell lysate stimulated peritoneal macrophages vaccinated group are significantly higher than in control groups.Cytokine proteins of IL-2, IL-4, IL-10 and IFN-γ were all successfully detected in spleen cells of each group.Conclusion: necrosis H22 cell lysate stimulated macrophage results effective cytolytic activity on H22 hepatocarcinoma cells and antitumor response.