【摘 要】
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UDP-Glucucunosyltransferase (UGT) is an important class of phase Ⅱ metabolizing enzymes, playing a pivotal role in detoxifying various substances and in the pathological procedures of some diseases.Th
【出 处】
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第十届全国药物和化学异物代谢学术会议暨第三届国际ISSX/CSSX联合学术会议
论文部分内容阅读
UDP-Glucucunosyltransferase (UGT) is an important class of phase Ⅱ metabolizing enzymes, playing a pivotal role in detoxifying various substances and in the pathological procedures of some diseases.The present study aims to uncover the potential dysregulation pattern of UGTs in trinitrobenzene suifonic acid (TNBS) induced colitis.Colitis was induced by intra-rectally administered with a single dose of TNBS (100 mg/kg).The expression and enzyme activity of hepatic UGTs of colitis rats were all down-regulated significantly except UGT1A7 for which the mRNA level was up-regulated.In contrast, UGT isoforms in the small intestine were relatively unaffected.In colon, where the inflammation occurs, the mRNA level and enzyme activity of UGT1 A1 and 1A6 were down-regulated, but those of UGT1A7 and 2B1 up-regulated.The mRNA levels of various transcription factors, including AhR, CAR, PXR, PPARγ, and FXR were all decreased, except for AhR and CAR in small intestine and colon.Our data suggests that colitis induces an isoform-dependent and tissue-specific dysregulation of UGTs and their related transcription factors.
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