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Aim: Compound K (CK) is a final intestinal metabolite of protopanaxadiol-type ginsenosides from Panax ginseng and shows various bioactivities.Although it has also been found to have the property of anti-diabetes, the long-term effect of CK on insulin secretion in β-cells is still unclear.In this study, CK was prepared from ginsenoside Rd by snailase hydrolysis and its effect on the insulin secretion activity in MIN6 pancreatic β-cell lines in vitro was assessed.