Aim We are interested in developing a well-controlled mechanical injury thrombosis mouse model that involves pinching of the aorta with forceps.Methods In this study, we established two levels of aortic injury induced by forceps pinching.Complete deformation of the endothelium was observed by SEM and TEM in the moderate injury model.Exposure of the media was achieved in the severe injury model.Platelets covered the denuded surface, and aggregates were detected.Results The arrival of a transient thrombus that peaked at approximately 1 min after injury and resolved within 3 min was observed in the moderate injury model, whereas a larger thrombus was observed in the severe injury model Furthermore, at least two peaks were observed in arteries with severe lesions.Administration of clinical medicaments decreased the formation of thrombi in these models.All of these lesions were sensitive to platelet GPⅡb-Ⅲa, P2Y1/P2Y12 inhibition and thrombin blockade.Aspirin only reduced the thrombus size in the severe injury model.This model will allow us to identify new anti-thrombotic targets for the treatment of thrombosis.Conclusions The model exhibited optimal reproducibility and confirmed the feasibility of inducing arterial thrombosis by mechanical injury Drugs targeted GP Ⅱ b-Ⅲa, ADP receptor P2Y1, P2Y12 and thrombin all appear to alleviate thrombosis in the thrombosis models.