Extract from Ginkgo biloba leaves has become a very popular plant medicine and herbal supplement for its potential benefit in alleviating symptoms associated with peripheral vascular disease，dementia，asthma and tinnitus．Most researches on G．biloba leaves focus on the leaves collected on July and August from the tree of 4-7 years，a large number of 1 eaves from fruit cultivars(the tree of a bove 10 years)are ignored and become obsolete after fruit harvest season (November)．In this paper，we expand the tree age range(from 1 to 300 years)and first comparatively analyze the total flavonol glycosides and terpene lactones in different ages，cultivation Sources and gender of G．biloba leaves collected in November by using the validated HPLC—ELSD and HPLC-PDA methods．The results showed that the contents of total terpene lactones and flavonol glycosides in the leaves of young ginkgo tree are higher than that in old tree，and they were higher in male trees than those in female．And geographical factors appear to have significant influence on their contents．All the results will provide good basis for comprehensive utilization of G．biloba leaves，especially the leaves from fruit cultivars．