目的：制备新型MIP-2γ/GM-CSF细胞因子融合蛋白，研究其趋化和激活DC的双重生物学功能.方法：通过基因工程方法构建高表达MIP-2γ/GM-CSF融合蛋白的2种P.pastoris酵母工程菌株，并优化发酵、纯化条件，制备了高纯度的MIP-2g/hGM-CSF、MIP-2g/mGM-CSF融合蛋白.首先以Transwell体系检测融合蛋白对树突状细胞DC的诱导迁移效应.接着以流式细胞仪分析重
Object: Tumor associated macrophages (TAMs) play critical roles in promoting tumor progression and invasion.However, the molecular mechanism for the regulation of TAM remains to be further investigate
Background and Purposes: A specific and strong T-cell response against HBV does not occur in patients with HCC.Adoptive T cell therapy can transfer therapeutic immunity.
Introduction: Colorectal cancer is a serious threat to human health.And metastasis is the key factor of poor prognosis.Recently, we have found that the negative costimulatory molecule B7-H3 is highly
目的：肿瘤发展与慢性炎症尤其是IL-23/IL-17通路相关。然而，人大肠癌组织IL-17的来源及其分泌细胞促进肿瘤进展的机制尚不清楚。方法：我们通过多色流式分析及原代细胞分离技术研究人大肠癌及配对组织IL-17的细胞来源及炎性细胞组成。
Inflammation is a critical component involved in tumor progression.IL-17 cytokine family, though still young since discovery, has recently emerged as important players in diseases.
Objectives:The inhibitor of DNA binding/differentiation 3 (Id3) protein is a helix-loop-helix transcription factor and might have an important role in cell proliferation and differentiation.
Objective: Pancreatic cancer is an aggressive malignancy and unresponsive to conventional radiation and chemotherapies.Therefore, the development of novel immune therapeutic strategies is urgently nee
Background Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide and ranks second among all the primary cancer-related mortalities.
目的：建立增龄性力学性能改变和磨耗状态下颌第一磨牙的三维有限元模型,用于分析老年磨耗牙的生物力学特点.方法：下颌前磨牙分年轻(17～25岁),中年(40～49岁),老年(65～80岁)三组,沿长轴颊舌向纵剖,取近中.原子力结合纳米压痕获得冠部牙本质和牙釉质,根颈、中、尖牙本质的弹性模量.利用CT数据建立下颌第一磨牙的有限元模型,牙合面分别由舌向颊侧、由近中向远中依次磨除0.5～1.5mm和2～3m