【摘 要】
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Luman/CREB3 has been identified as a novel transcriptional co-regulator of a variety of nuclear receptor.We report herein for the first time that Luman is expressed in male mouse testis.Immunocytochem
【机 构】
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Key Laboratory of Animal Biotechnology of the Ministry of Agriculture,Northwest A&F University,Yangl
【出 处】
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中国畜牧兽医学会兽医产科学分会第十三次学术研讨会
论文部分内容阅读
Luman/CREB3 has been identified as a novel transcriptional co-regulator of a variety of nuclear receptor.We report herein for the first time that Luman is expressed in male mouse testis.Immunocytochemical and real-time PCR results showed positive Luman immunostaining in Leydig cells and the expression pattern of Luman in mouse testis exhibited an age-related increase.Immunofluorescent staining of Luman in the MLTC-1 Leydig cell line indicates that Luman is located within the cytoplasm.HCG significantly up-regulated the levels of expression of Luman in MLTC-1 cells and mouse testes,respectively.To elucidate the function of Luman, experiments were conducted to examine the consequences of lentiviral-mediated RNA interference (RNAi) of Luman on testosterone production and expression of several genes involved in steroidogenesis.Luman silencing caused a increase in HCG-stimulated testosterone production.In addition, Luman silencing significantly up-regulated the mRNA levels of P450scc and P450c17 in primary Ledyig cells and MLTC-1 cells.Overall, our data are the first to provide evidence for a possible direct action of Luman in the control of testicular function.Together, these data indicate that the expression of Luman in mouse testis is stage dependent and androgen inductive, and suggest the important role of Luman in male reproduction.
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