2. 您的位置
3. 首页
4. 会议论文
5. An Integrated Device with Combination of Protein Denaturation, Reduction, Desalting and on-column Di

An Integrated Device with Combination of Protein Denaturation, Reduction, Desalting and on-column Di

来源 :第七届中国蛋白质组学大会暨第三届国际蛋白质组学论坛 | 被引量 : 0次 | 上传用户：mynewgolvoe

【摘 要】

：

For proteome research,"bottom-up" strategy is one of the most commonly used approaches,by which proteins are in turn denatured,reduced,alkylated,desalted and di

【作 者】

：

Huiming Yuan Hao Jiang Zhongpeng Dai Lihua Zhang Zhen Liang Yukui Zhang 

【机 构】

：

KeyLaboratoryofSeparationScienceonAnalyticalChemistry,NationalChromatographicR.&.A.Center,DalianInst

【出 处】

：

第七届中国蛋白质组学大会暨第三届国际蛋白质组学论坛

【发表日期】

：

2011年4期

【关键词】

：

On-line protein pretreatment Integrated device membrane interface IMER Human ser 

下载到本地 , 更方便阅读

下载此文 赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

For proteome research,"bottom-up" strategy is one of the most commonly used approaches,by which proteins are in turn denatured,reduced,alkylated,desalted and digested prior to multidimensional HPLC-ESI/MS/MS analysis.

其他文献

A Strategy for Peptidomic Marker Candidate Verification Using Peptide Antibodies and MALDI-TOF-MS

Serum peptides were the rich source for cancer biomarker development.Much works focused on the peptide marker discovery in the past ten years,but the verificati

会议

Hepatocellular carcinomapeptidomicsantibodyMALDI-TOF-MS

Preparation of a Series of Novel Peptide-PEG-drug Conjugates for Small-molecule Bioactive Compound T

The interactions between small-molecule bioactive compounds and their target proteins have been found to be more promiscuous than originally anticipated,which c

会议

Target profilingchemical proteomicspoly (ethyl glycol)mass spectrometryaffin

Differential Regulation of OmpC and OmpF by OmpREnvZ and CpxACpxR in Response to Nalidixic Acid and

The regulation mechanisms by which OmpR/EnvZ and CpxA/CpxR manipulate the OmpC and OmpF in resistance to antibiotics are still largely unknown.

会议

Escherichia coliouter membrane proteinstwo-component systemsantibiotic resist

Involvement of BAG2 in c-Myc Suppression Induced Cell Senescence

Suppression of c-Myc oncogene was reported to prompt cell senescence leading to carcinogenetic arrest in many tumors.Previous observations suggested that BCL2-a

会议

BAG2c-MycSenescenceChIP

Regular Patterns for the Selective Expression of Genome Characterized by Protein Domain Architecture

Due to the space- and stage- specific pattern of gene regulation,the gene expression profiles at different physiological states are quite specific.However,there

会议

Domainproteomegenomebiological complexity

Identification of a New Set of Immunohistochemical Biomarkers for Classification of Dysplastic Nodul

BACKGROUND:The study aims to screen immunohistochemical biomarkers for distinguishing diagnosis of small hepatocellular carcinoma (sHCC) from dysplastic nodules

会议

iTRAQ-2DLC-ESI-MS/MSDysplastic Nodulessmall Hepatocellular CarcinomaAminoacyl

Hippocampus Proteins Quantification and Interaction Analysis of Transgenic Mouse Encoded with Double

We hypothesize that the study of the global protein changes that occur in very specific regions of the brain will provide a better understanding of the processe

会议

Alzheimer’s diseasehippocampusproteomicspathwayinteraction

Preparation of a Multi-layer Open Tubular Capillary Column Based Immobilized Enzyme Reactor and Its

Trypsin is currently the most widely adopted protease for protein digestion in shotgun proteomic studies.Though effectively used both in the digestion of simple

会议

Open-tubular capillaryImmobilized trypsin reactorProtein digestionDendritical

Dynamic Responses of the Rice Embryo Proteomes to Embryogenesis the Proteomic Analysis to the Embryo

Plant embryo is the germinate center of seed.However,there is still lack of full understanding of how an embryo forms during seed maturation,especially for mono

会议

RiceEmbryoEmbryogenesisProteomicsGlobulinProtein interaction

An Integrated High-throughput Workflow for Identification of Crosslinked Peptides from Complex Sampl

Introduction:Chemical crosslinking combined with mass spectrometry (xMS) is a potentially powerful tool for protein interaction studies and protein structural d

会议