【摘 要】
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Objective To investigate the intracellular mechanism of proBDNF on neuron by detecting expression of ELK-p,ERK and c-fos in cultured hippocampal neuron.Meth
【机 构】
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Department of Human Anatomy and Neurobiolog, Xiangya School of Medicine, Central South University, C
【出 处】
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中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
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Objective To investigate the intracellular mechanism of proBDNF on neuron by detecting expression of ELK-p,ERK and c-fos in cultured hippocampal neuron.Methods The hippocampal neurons were dissociated from El8 rats and cutured in neurobasal medium.Then the cells were treated with proBDNF,preBDNF and proBDNF antiserum,respectively.Thirty minutes,1 h or 48 h later,the cells were stained with Nissl solution and ELK-p,ERK2,c-fos immunocytochemistry were performed.Results It showed that the expression of ELK-p,ERK and c-fos was significantly upregulated in cultured hippocampal neuron after treated with proBDNF protein,and sometimes the immunoreactivity was seen in some nucleous.Whereas the expression was downregulated with proBDNF antiserum treatment,and many cells exhibited swelling and vasoculation.The immunoreactivity in preBDNF treated cells was similar to that in normal cultured cells.Conclusion It seems that proBDNF plays an important role in sustaining the hippocampal neuron survival through upregulating the ELK and ERK pathway.
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