The first commercial next-generation sequencer (NGS) has born nearly 10 years.The technology had become one of the most exciting and rapid-developing fields with tremendous competition and great influences in both industrial and academic fields of biomedical engineering during the past 10 years.Nowadays, the single DNA sequencing named as the 3rd generation sequencing technology, is attracting many attentions, which could be featured as single long DNA sequencing at much low cost and high speed without DNA amplification.