【摘 要】
：
Populus euphratica is one of the most important timber species in the north of China.As the unique Chinese character of poplar, it is known for its high salt tolerance.One binary bacterial artificial
【机 构】
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Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Be
【出 处】
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中国植物学会植物细胞生物学2012学术年会
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Populus euphratica is one of the most important timber species in the north of China.As the unique Chinese character of poplar, it is known for its high salt tolerance.One binary bacterial artificial chromosome (BIBAC) library was constructed which represents the first plant-transformation-competent large-insert DNA library produced for Populus euphratica.The BIBAC library was constructed in BamHI site of binary vector pCLD04541 by ligation of partial digested nuclear DNA of Populus euphratica.This library consists of 55,296 clones with an average insert size of 85 kb, and covers over 40 folds Populus euphratica haploid genomes.BIBAC clones which contain inserts from 50 kb to 150Kb were selected and transformed into Arabidopsis for salt tolerant plant screening.One transgenic line was found to be more salt tolerant than wild type from the screen of 150 lines in 175mM NaC1.It was demonstrated that the clone contains candidates of salt tolerance genes and the approach is suitable for the transformation of salt sensitivity lumbers for genetic improvement.
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