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Aims: Induced pluripotent stem cells (iPSs) are believed to have great therapeutic potential.Treatment for acute kidney injury is challenging.Therefore,in the present study,we aimed to demonstrate if iPSs attenuate acute kidney injury and the possible mechnisms,especially the role of reactive oxygen species (ROS).Methods: In vivo experiments: C57BL/6 mice were subjected to right nephrectomy,and left renal ischemia for 50min,then resuscitated with Ringer’s lactate.After the operation,the mice were injected iPSs with or without NAC pre-treated via the tail vein.The kidney tissue and serum were collected 24 h,48h after resuscitation.Creatinine,pathology score,cytokine expression,iPSs quantification and identification in injury kidney were detected.In vitro EC- iPSs experiments: mice glomerular endothelial cells isolated from C57BL/6 were pre-treated with MCP-1 4h,and then added iPSs with or without NAC treated.Following that the adhesion and transendothelial migration of IPS were detected.