【摘 要】
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Aim To evaluate the antiinflammatory effects of ethanol fraction prepared from Disporum cantoniense(Lour.) Merr.70% ethanol extract with a cellular model of LPSstimulated RAW264.7 cell.Methods RAW264.
【机 构】
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Guizhou Provincial Key Laboratory of Pharmaceutics,School of Pharmacy,GuiZhou Medical University,Gui
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Aim To evaluate the antiinflammatory effects of ethanol fraction prepared from Disporum cantoniense(Lour.) Merr.70% ethanol extract with a cellular model of LPSstimulated RAW264.7 cell.Methods RAW264.7 cells were treated with different concentrations of ethanol fraction (25, 50 and 100 g.L1) and stimulated with LPS (10 μg · L1) for 24 hours, the conditioned media was collected and analyzed.The quantity of nitric oxide (NO) was assayed by Griess reagent.The production of inflammatory mediators was determined by enzymelinked immunosorbent assay (ELISA), such as prostaglandin E2 (PGE2), tumor necrosis factor o (TNFot), interleukin1β (IL1β) and interleukin 6 (IL6) in cell supernatant.The concentrations of inflammatory mediators were calculated according to the standard curves generated by each of the recombinant cytokines provided with the ELISA kits.Results Compared with the control group, LPS can induce RAW264.7 cells to promote the production of inflammatory mediators (P <0.01), including NO, PGE2, TNFα, IL1β and IL6.Compared with the model group, ethanol fraction significantly suppressed LPS induced release of inflammatory mediators such as nitric NO, PGE2, TNFα, IL1 β and IL6 in a good dose dependent manner (P < 0.05, P < 0.01).Conclusions Ethanol fraction could significantly inhibit the production of LPSinduced inflammatory response in RAW264.7 cells,and its antiinflammatory effect may be related to reduce the production of inflammatory mediators NO, PGE2, TNFα, IL1 β and IL6.These results demonstrate that the ethanol fraction is the bioactive component of Disporum cantoniense (Lour.) Merr., and the ethanol fraction will be further developed as a herbal remedy for preventive and/or curative purposes in various inflammatory diseases.
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