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目的研究维康醇对肝癌细胞增殖、细胞周期和细胞凋亡作用的影响。方法以不同浓度维康醇处理对数生长期人肝癌细胞HepG2,利用MTT法测定维康醇对细胞的增殖抑制作用,流式细胞仪PI单染检测细胞周期分布情况的改变,流式细胞仪Annexin V FITC/PI双标记法检测细胞凋亡率。结果 MTT结果显示:维康醇对HepG2细胞具有明显的生长抑制作用并呈浓度和时间依赖性。流式细胞术结果显示:30μmol/L维康醇作用于HepG2细胞48 h后,处于G2/M期的细胞比例显著升高,同时伴随G1/G0期细胞比例下降,与对照组相比差异有统计学意义(P<0.05)。Annexin V FITC/PI染色检测结果显示:30μmol/L维康醇作用于HepG2细胞48 h后,细胞凋亡率显著升高,与对照组相比差异有统计学意义(P<0.05)。结论维康醇对肝癌细胞有明显的增殖抑制作用,并具有浓度和时间依赖性;维康醇通过引起细胞周期阻滞、诱导细胞凋亡发挥其抗肝癌作用。
Objective To study the effects of vevitol on the proliferation, cell cycle and apoptosis of hepatocellular carcinoma cells. Methods HepG2 cells were treated with different concentrations of vevitol in logarithmic growth phase. The proliferation of HepG2 cells was assayed by MTT assay. Cell cycle distribution was analyzed by flow cytometry with PI single staining. Flow Cytometry Annexin V FITC / PI double labeling method to detect apoptosis rate. Results The results of MTT showed that vevitol had a significant growth inhibitory effect on HepG2 cells in a concentration- and time-dependent manner. The results of flow cytometry showed that the proportion of cells in G2 / M phase was significantly increased after treated with 30μmol / L veconitol for 48 h, while the proportion of cells in G1 / G0 phase decreased compared with control group Statistical significance (P <0.05). The results of Annexin V FITC / PI staining showed that the apoptotic rate of HepG2 cells treated with 30 μmol / L veconitol for 48 h was significantly higher than that of the control group (P <0.05). CONCLUSION: Veconn can inhibit the proliferation of hepatocellular carcinoma cells in a concentration-dependent and time-dependent manner. Veconazole exerts its anti-hepatocellular carcinoma effect by inducing cell cycle arrest and inducing apoptosis.