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Aim:To investigate the neuroprotective effect and mechanisms of scutellarin,aflavonoid extracted from Erigeron breviscapus Hand Mazz,against neuronal dam-age following cerebral ischemia/reperfusion.Methods:Rats were pretreated igwith scutellarin for 7 d and then subjected to cerebral ischemia/reperfusion(I/R)injury induced by a middle cerebral artery occlusion(MCAO).The infarct volumeand neurological deficit were determined by TTC staining and Longa’ s score.Thepermeability of the blood-brain barrier was evaluated by measurement of the Evansblue(EB)content in the brain with a spectrophotometer.The total NOx contentwas determined.Nitric oxide synthase(NOS)isoforms(iNOS,eNOS,nNOS)andthe key angiogenic molecules,vascular endothelial growth factor(VEGF)andbasic fibroblast growth factor(bFGF),were detected by Western blotting.Results:Scutellarin significantly reduced infarct volume(P<0.05 or P<0.01),amelioratedthe neurological deficit and reduced the permeability of the blood-brain barrier(BBB)(P<0.05).When rats were pretreated with scutellarin(50 or 75 mg/kg),upregulation of eNOS expression and downregulation of VEGF,bFGF,and iNOSexpression was observed,whereas scutellarin had no effect on nNOS expression.Conclusion:Scutellarin has protective effects for cerebral injury through regulat-ing the expression of NOS isoforms and angiogenic molecules.
Aim: To investigate the neuroprotective effect of mechanisms of scutellarin,aflavonoid extracted from Erigeron breviscapus Hand Mazz,against neuronal dam-age following cerebral ischemia/reperfusion.Methods: Rats were pretreated igwith scutellarin for 7 d and then term to cerebral ischemia/reperfusion ( I/R) injury induced by a middle cerebral artery occlusion(MCAO).The infarct volumeand neurological deficit were determined by TTC staining and Longa’s score.Thepermeability of the blood-brain barrier was evaluated by measurement of the Evansblue(EB)content In the brain with a spectrophotometer.The total NOx contentwas determined.Nitric oxide synthase(NOS)isoforms(iNOS,eNOS,nNOS)andthe key angiogenic molecules,vascular endothelial growth factor(VEGF)andbasic fibroblast growth factor(bFGF),were detected by Western blotting. Results: Scutellarin significantly reduced the farlogical volume (P<0.05 or P<0.01), ameliorated the neurological deficit and reduced the permeability of the blood-brain barrier ( BBB) (P<0.05). When rats were treated with scutellarin (50 or 75 mg/kg), upregulation of eNOS expression and downregulation of VEGF, bFGF, and iNOSexpression was observed, whereas scutellarin had no effect on nNOS expression.Conclusion: Scutellarin has protective effects for cerebral injury through regulat-ing the expression of NOS isoforms and angiogenic molecules.