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目的:探讨促红细胞生成素(EPO)在缺铁性贫血(IDA)大鼠模型中对铁调素(hepcidin)表达的影响。方法:通过反复放血和饲喂低铁饲料,建立IDA大鼠动物模型,比较药物干预组(EPO)与非干预组及正常对照组与正常给药组(EPO)大鼠血清hepcidin表达的差异。Western blot检测肝脏内信号因子CCAAT/增强子结合蛋白α(C/EBPα)含量。结果:IDA模型组腹腔注射EPO后,血清hepcidin和肝脏C/EBPα含量显著降低。EPO对正常大鼠hepcidin表达也有显著抑制作用(P<0.05)。结论:hepcidin可能参与了IDA的发生,在正常大鼠和IDA大鼠中,EPO能通过下调C/EBPα阻碍其激活hepcidin启动子最终抑制hepcidin的表达。
Objective: To investigate the effect of erythropoietin (EPO) on hepcidin expression in a rat model of iron deficiency anemia (IDA). Methods: The animal model of IDA was established by repeatedly excreting blood and feeding iron-deficient diet. The differences of serum hepcidin expression between drug-treated group (EPO) and non-intervention group, normal control group and normal control group (EPO) rats were compared. The levels of CCAAT / enhancer binding protein α (C / EBPα) in the liver were detected by Western blot. Results: After intraperitoneal injection of EPO in IDA model group, the contents of hepcidin and C / EBPα in liver decreased significantly. EPO also had a significant inhibitory effect on hepcidin expression in normal rats (P <0.05). CONCLUSIONS: Hepcidin may be involved in the development of IDA. In normal rats and IDA rats, EPO can inhibit the expression of hepcidin by inhibiting the activation of hepcidin promoter by down-regulating C / EBPα.