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目的观察氟中毒病区人群抗氧化能力及过氧化产物水平,为筛选氟中毒早期诊断指标提供依据。方法选取贵州省六盘水市陡箐乡氟病区165人为病区组,同市非病区花嘎乡90人为对照组,经体检及氟斑牙判定后采集空腹血液、尿液,分别检测尿氟、血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-Px)、总抗氧化能力(T-AOC)活力,同时检测血清中丙二醛(MDA)、8-异前列腺素F2α(8-isoPGF2α)、晚期氧化蛋白产物(AOPPs)和8-羟基脱氧鸟苷(8-OHdG)含量,分析氧化应激指标与氟斑牙及尿氟水平的相关性。结果病区组尿氟高于对照组(P<0.05),SOD、GSH-Px活力低于对照组(P<0.05),且与氟斑牙程度均呈负相关(P<0.05)。病区组血清中8-iso-PGF2α高于对照组(P<0.05),与氟斑牙程度呈正相关(P<0.05);病区组成人血清中AOPPs、8-OHdG高于对照组(P<0.05),但病区与非病区组儿童间比较,差异无统计学意义(P>0.05)。结论氟中毒病区人群普遍处于氧化应激状态,并存在脂质生物膜、蛋白质及DNA的多种损伤。
Objective To observe the anti-oxidative capacity and the level of peroxidation products in people with fluorosis and to provide the basis for screening early diagnosis of fluorosis. Methods A total of 165 patients with fluorosis in Stefan Township, Liupanshui City, Guizhou Province, were selected as the control group. 90 patients in Huajia Township, the same city and non-diseased area as the control group. Fasting blood and urine were collected after physical examination and dental fluorosis determination. Urinary fluoride, Serum levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) were measured. Serum malondialdehyde (MDA), 8-isoprostane F2α (8-isoPGF2α), advanced oxidation protein products (AOPPs) and 8-hydroxy-deoxyguanosine (8-OHdG) were measured. The correlation between oxidative stress and dental fluorosis and urinary fluoride were analyzed. Results The urinary fluoride in the ward was significantly higher than that in the control group (P <0.05). The activities of SOD and GSH-Px in the ward were lower than those in the control group (P <0.05) and negatively correlated with the dental fluorosis (P <0.05). The serum 8-iso-PGF2α in the ward was higher than that in the control group (P <0.05), and positively correlated with the degree of dental fluorosis (P0.05). The serum AOPPs and 8-OHdG in the ward were higher than those in the control group <0.05), but there was no significant difference between children in ward and non-ward group (P> 0.05). Conclusion The population of fluorosis area is generally oxidative stress, and there are many damage of lipid biomembrane, protein and DNA.