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为了研究丹参中特有的NAC转录子在丹参生长发育、激素调节和抗逆胁迫应答调节中的功能,对丹参NAC转录因子进行了克隆和分析。根据丹参毛状根cDNA文库中筛选到的NAC EST序列,克隆了丹参SmNAC1的cDNA全长序列。生物信息学分析显示基因的开放阅读框591 bp,编码166个氨基酸,相对分子质量21.66 kDa,等电点4.36 Genbank kF006346。SmNAC1蛋白的N-端具有保守的NAC_AB结构域,C-端高度变异。根据软件预测SmNAC1可能定位在细胞核。qRT-PCR分析YE+Ag+处理后SmNAC1在丹参毛状根中的表达变化,处理后2 h表达量上调至对照的1.5倍,4～12 h保持2倍的表达量,36 h时下降至对照水平以下,推测SmNAC1可能参与了丹参毛状根对YE+Ag+的胁迫应答调节。
In order to study the function of Danshen NAC transcriptional promoter, which is characteristic of Salvia Miltiorrhiza, in the regulation of growth and development, hormone regulation and stress tolerance response in Salvia miltiorrhiza, the NAC transcription factor of Danshen was cloned and analyzed. According to the NAC EST sequence screened from the hairy root cDNA library of Salvia miltiorrhiza, the full-length cDNA sequence of SmNAC1 was cloned. Bioinformatics analysis showed that the open reading frame of the gene was 591 bp, encoding 166 amino acids with a relative molecular mass of 21.66 kDa and an isoelectric point of 4.36 Genbank kF006346. The N-terminal of SmNAC1 protein has a conserved NAC_AB domain with a high degree of C-terminal variation. According to the software, SmNAC1 may be located in the nucleus. The expression of SmNAC1 in hairy roots of Salvia miltiorrhiza Bunge after YE + Ag + treatment was analyzed by qRT-PCR. The expression of SmNAC1 was up-regulated 1.5-fold at 2 h and 2-fold at 4-12 h after treatment, and decreased to control at 36 h Level below, speculated SmNAC1 may be involved in the Salvia miltiorrhiza hairy roots YE + Ag + stress response regulation.