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用微量组织培养塑胶板培养细胞,在显微镜下观察细胞形态变化,可以研究药物对细胞的影响、病毒感染引起的细胞病变以及药物抗病毒、抗肿瘤的活性,并能定量测出病毒的半数感染量(TCID_(50))、药物50％中毒剂量(CTD_(50))和药物50％有效剂量(ED_(50))等,方法简便经济,用药量小,短期内可得出系列性结果,为实验室常用方法。但仅根据形态变化,判断细胞病变程度,难于避免主观因素,以致结果有时波动,不易获得可重复的定量数据。
Micro-tissue culture plastic plates cultured cells, observed under a microscope cell morphology changes, you can study the impact of drugs on cells, viral infections caused by cytopathicity and drug anti-virus, anti-tumor activity, and quantitative detection of the virus half of the infection (TCID 50), 50% toxic dose (CTD 50) and 50% effective dose (ED 50) of the drug, the method is simple and economical, the dosage is small, and the serial results can be obtained in a short time, Common method for the laboratory. However, only based on morphological changes, to determine the degree of cytopathic effect, it is difficult to avoid subjective factors, resulting in fluctuations in the results sometimes difficult to obtain reproducible quantitative data.