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采用改进的Hummers方法制备氧化石墨烯(GO),再用共沉淀法原位合成Fe3O4磁性纳米粒子修饰的GO复合材料。通过透射电镜(TEM)、傅里叶变换红外光谱(FT-IR)和X-射线衍射(XRD)对Fe3O4纳米粒子-GO复合物的形态进行表征。结果表明,所制复合物中Fe3O4磁性纳米粒子的粒径为30 nm,少量粒子团聚后的粒径为50～100 nm。该复合物中GO含有多种官能团,可作为药物连接的位点,还可通过-共轭作用与药物连接。在外加磁场作用下,可明显观察到该复合物迅速聚集在永磁体附近。体外细胞毒性试验表明,该复合物在0.5～5 000 g/ml范围内对肺癌A549细胞和乳腺癌ZR-75-30细胞无细胞毒性。
The graphene oxide (GO) was prepared by the modified Hummers method and the GO composite modified with Fe3O4 magnetic nanoparticles was synthesized in situ by coprecipitation method. The morphology of Fe3O4 nanoparticle-GO composite was characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) The results show that the particle size of the Fe3O4 magnetic nanoparticles in the prepared composite is 30 nm and the particle size of the small particles is 50-100 nm. The complex GO contains a variety of functional groups, which can be used as a site for drug attachment, and can also be linked to the drug by-conjugation. Under the action of an applied magnetic field, it is clearly observed that the complex rapidly accumulates near the permanent magnet. In vitro cytotoxicity assay showed that the complex was non-cytotoxic to lung cancer A549 cells and breast cancer ZR-75-30 cells in the range of 0.5-5 000 g / ml.