论文部分内容阅读
本文介绍两种简单、灵敏的测定青霉素类药物的方法。原理是青霉素作为π-电子给予体,2,3-二氯-5,6-二氰对苯醌(DDQ)或7,7,8,8-四氰对醌二甲烷(TCNQ)为π-接受体,两者相互反应定量生成深色阴离子基,用分光光度法测定。用这两种方法分别测定了苄青霉素、无水氨苄青霉素、氨苄青霉素钠、苯唑青霉素、羟氨苄青霉素和甲氧苯青霉素等6种药品的纯品和制剂型药品。DDQ和TCNQ法给出标示量的平均标准偏差分别为99.12%±1.59%～100.65%±1.72%和98.92%±1.09%～101.30%±0.85%。这两种方法比法定法简单、省时和灵敏。DDQ法还可用于当青霉素裂解产物和硫酸链霉素存在时青霉素的常规测定。
This article describes two simple and sensitive methods for the determination of penicillins. The principle is that penicillin is a π-electron donor, and 2,3-dichloro-5,6-diancyanoquinone (DDQ) or 7,7,8,8-tetracyanoquinodimethane (TCNQ) Recipient, the two react with each other quantitative generation of dark anion, measured spectrophotometrically. The two methods were used to determine the pure and preparation medicines of 6 kinds of medicines, such as benzyl penicillin, anhydrous ampicillin, ampicillin sodium, oxacillin, amoxicillin and methicillin. The average standard deviations of DDQ and TCNQ were 99.12% ± 1.59% ~ 100.65% ± 1.72% and 98.92% ± 1.09% ~ 101.30% ± 0.85%, respectively. These two methods are simple, time-saving and sensitive to statutory law. The DDQ method can also be used for the routine determination of penicillin in the presence of penicillin lysate and streptomycin sulfate.