抗菌素的定量测定是药物分析中较困难的一个领域。微生物、分光光谱和化学方法尚歉专一性。气相层析仅用于对热稳定的抗菌素,而高速液相层析则限于四环素类,头孢菌素 C 等的测定。反相高速液相层析优于上述方法,与离子交换层析相比,其优点为:①移动相简单(一般为甲醇,乙腈,四氢呋哺或二氧六环的水溶液);②使用无腐蚀性的盐类; Antimicrobial quantification is one of the more difficult areas in drug analysis. Microbiological, spectroscopic and chemical methods are still one-of-a-kind. Gas chromatography is only used for heat-stable antibiotics, while high-speed liquid chromatography is limited to the determination of tetracyclines, cephalosporin C and the like. Reversed-phase high-speed liquid chromatography is superior to the above method, compared with ion exchange chromatography, its advantages are: ① mobile phase is simple (usually methanol, acetonitrile, tetrahydrofuran or dioxane aqueous solution); ② use Non-corrosive salts;