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目的:观察复方土茯苓颗粒对高尿酸血症肾病模型小鼠血尿酸、肾功能的影响及肾脏组织中白细胞介素1(IL-1β)、白细胞介素6(IL-6)表达的影响。方法:采用腺嘌呤(100 mg.kg-1)联合97%氧嗪酸钾(1 g.kg-1)ig法建立小鼠高尿酸血症肾病动物模型,设立正常对照组、造模组、阳性药物组苯溴马隆组20 mg.kg-1、复方土茯苓颗粒高、中、低剂量组(6,3,1.5 g.kg-1)。实验第21天小鼠眼球采血后处死,检测血清中尿酸、肌酐、尿素氮及肾组织中IL-1β,IL-6水平。结果:复方土茯苓颗粒高、中、低剂量组尿酸(110.22±28.07),(128.10±54.79)(144.10±51.73)μmol.L-1;肌酐(39.11±8.55),(60.90±21.29),(87.50±16.18)μmol.L-1;尿素氮(25.15±3.17),(29.65±2.17),(35.01±3.63)mmol.L-1;IL-1β(3.13±0.53),(4.26±1.44),(5.10±1.86)μg.L-1;IL-6(14.90±4.93),(22.87±6.71),(35.83±22.19)μg.L-1;与模型组血尿酸(259.22±31.05)μmol.L-1、肌酐(103.00±22.34)μmol.L-1,尿素氮(50.76±9.93)mmol.L-1,IL-1β(10.74±4.0)μg.L-1,IL-6(62.87±15.42)μg.L-1相比明显降低(P<0.05),复方土茯苓颗粒高、中剂量组与苯溴马隆组相比,组间比较无统计学差异。结论:复方土茯苓颗粒能够有效降低高尿酸血症肾病模型小鼠尿酸、肌酐、尿素氮水平,保护尿酸造成的肾脏损伤,可能机制为降低IL-1β,IL-6等炎症因子的表达。
Objective: To observe the effect of Fufang Tu Fuling granule on serum uric acid and renal function in hyperuricemic nephropathy model mice and the expression of interleukin-1β (IL-6) and interleukin-6 (IL-6) in kidney tissue. Methods: The animal model of mouse hyperuricemia with nephropathy was established by adenine (100 mg.kg-1) combined with 97% oteracil potassium (1 g.kg-1) ig and the normal control group, model group, Positive drug group Benzbromarone group 20 mg.kg-1, compound Tufuling granules high, medium and low dose groups (6,3,1.5 g.kg-1). Blood was collected from the eyeballs of mice on day 21 after the experiment, and serum levels of uric acid, creatinine, blood urea nitrogen and renal tissue were measured for IL-1β and IL-6 levels. Results: The contents of uric acid (110.22 ± 28.07), (128.10 ± 54.79) (144.10 ± 51.73) μmol.L-1, creatinine (39.11 ± 8.55), (60.90 ± 21.29), 87.50 ± 16.18) μmol·L-1, urea nitrogen (25.15 ± 3.17), (29.65 ± 2.17), (35.01 ± 3.63) mmol.L-1, IL-1β (3.13 ± 0.53) (5.10 ± 1.86) μg.L-1, IL-6 (14.90 ± 4.93), (22.87 ± 6.71) and (35.83 ± 22.19) μg.L-1, respectively. The levels of serum uric acid in the model group were (259.22 ± 31.05) (10.00 ± 22.34) μmol·L -1, urea nitrogen (50.76 ± 9.93) mmol·L -1, IL-1β 10.74 ± 4.0 μg.L-1, IL-6 62.87 ± 15.42, (P <0.05). Compared with benzbromarone group, there was no significant difference between the two groups in high and medium dose Fufang Tuckahoe Granules. CONCLUSION: Fufang Tuckahoe Granule can effectively reduce the level of uric acid, creatinine and urea nitrogen and protect the kidney from uric acid in hyperuricemic nephropathy model mice. The possible mechanism is to decrease the expression of IL-1β, IL-6 and other inflammatory factors.