论文部分内容阅读
在人工气候箱内 ,以小麦为寄主建立了专性寄生禾谷多粘菌 (Polymyxagraminis)的侵染体系 ,使P .graminis能够快速大量繁殖 ,生活史缩短为 1 3～ 1 5d。简化了单孢子堆分离以及病根表面消毒等分离纯化方法 ,对接种菌源材料、寄主苗龄、温度、pH值及营养液成分等影响因素进行了测定 ,优化完善了P .graminis的砂培条件。建立了针对小麦黄花叶病毒(wheatyellowmosaicvirus,WYMV)的稳定高效室内传毒体系。在此体系上 ,真菌传带病毒的效率可达 70 % ,机械接种病毒后的小麦显症时间可缩短至 30d左右。
In the artificial climate chamber, the infection system of Polymyxa brasiliensis was established by using wheat as its host, which allowed P.graminis to multiply rapidly and its life span was shortened to 13 ~ 15 days. The separation and purification methods such as single spore-pile separation and diseased root surface were simplified, and the influencing factors such as seedling age, temperature, pH value and nutrient composition of the inoculation were determined, and the sand culture conditions of P. Graminis . A stable and efficient indoor poisoning system against wheat yellow mosaic virus (WYMV) was established. In this system, the efficiency of fungal transmission of up to 70% of the virus, mechanical virus inoculation of wheat significantly reduce the time can be shortened to about 30d.